Exact(6)
The influence of [19F]BF4 − dose on biodistribution in vivo was evaluated in normal mice by nanoPET imaging and ex vivo tissue counting.
We further investigated [ 18 F]flutemetamol binding to different brain regions and structures inside the head in WT male C57Bl/6N mice (N = 4) using ex vivo tissue counting to better understand the observed binding in vivo.
We observed high radioactivity in close proximity to the brain in vivo, and subsequent ex vivo tissue counting experiments confirmed that significant radioactivity was located outside of the brain, inside the nasal cavity.
As expected, significant uptake of the radiotracer in the thyroid, salivary glands and stomach was observed both by PET/CT (30 min post-injection, Fig. 2) and by ex vivo tissue counting (45 min post-injection, Fig. 3, shown as %ID/g in Additional files 4 and 5), with tracer excretion proceeding via the renal route.
Mice were dissected and the major thoraco-abdominal organs, salivary glands, thigh muscle and femora collected for ex vivo tissue counting.
To assess early tissue distribution ten mice were inoculated via the tail vein with 2 × 10 cells labelled with 0.6 0.8 MBq [Zr]oxinate4 in 100 μl sterile PBS and culled by cervical dislocation at 9 (n = 3), 24 (n = 4) and 48 h (n = 3) post-injection for ex vivo tissue counting.
Similar(2)
The percent injected activity per gram (%IA g− 1) for each tissue was calculated by comparison of the tissue counts to a standard sample prepared from the injectate.
All follicle counts were then expressed as number of follicles counted per mm3 of ovarian tissue counted.
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