The hunger for tissue-clearing techniques originated with neuroscientists, who were frustrated by their limited ability to trace the snaking routes of axons and dendrites in the brain.
We compared the quality of immunostaining on post-mortem human brain tissues using several clearing techniques including CLARITY [ 4, 5], Scale [ 8], SeeDB [ 9], and 3DISCO [ 6]. Results were best with CLARITY.
Both Boyden and Deisseroth have founded companies to advance clinical development of their techniques, and some practitioners of tissue clearing already foresee the beginning of the end for conventional methods.
The optical clearing effect is the determination of optical backscattering or reflectance from the internal bio-tissue, so the most prospective applications for the optical clearing technique are expected in OCT.
Some are tissue-clearing methods that make opaque tissues transparent, whereas others complement tissue clearing with a proportional size increase that exposes molecular details to conventional microscopy.
It is considered that optical clearing technique can effectively reduce the multiple scattering of light and improve the effect of optical diagnostics of tissue [ 2, 6].
Richardson, D. S. & Lichtman, J. W. Clarifying tissue clearing.
Tissue clearing method and immunohistochemical staining of sucklings brain.
He also organizes an annual workshop to teach tissue clearing methods to MCB students.
Premise of the study: Due to lack of success in clearing whole tissues using only classical clearing techniques (e.g., Herr's 4½ solution, KOH, NaOH, lactic acid saturated with chloral hydrate), and because tissue degradation is often a result of harsh clearing agents (e.g., KOH, NaOH), a novel combined treatment was sought to improve the removal of obscuring tannins from intact haustoria.
In addition, some studies have been done on better understanding of the mechanism of tissues and blood optical clearing techniques.
