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Richardson, D. S. & Lichtman, J. W. Clarifying tissue clearing.
He also organizes an annual workshop to teach tissue clearing methods to MCB students.
The tissues were incubated with indicated Alexa dye-conjugated secondary antibodies diluted (1 500) in PBS/0.2% Tween-20/10 Tween-20/10in/5% donkey serum at 37 °C for 72 h, and washed in PBS/0.2% Tween-20/10 μg/mL heparin/5% 37 °C for 2 h five times before the tissue clearing.
Two properties of AP histochemistry are particularly useful for the morphologic analysis of large neurons: (1) efficient diffusion of the low-molecular weight histochemical substrates into fixed tissue and (2) the stability of the AP reaction product, which survives immersion in the tissue clearing agent benzylbenzoate∶benzyl alcohol (BBBA).
After overnight GUS staining and tissue clearing the number of GUS stained cells was determined and the percentage of GUS positive cells relative to the transformed cells calculated.
Some are tissue-clearing methods that make opaque tissues transparent, whereas others complement tissue clearing with a proportional size increase that exposes molecular details to conventional microscopy.
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In critically ill septic patients, after the initial resuscitation phase, adipose tissue clears lactate earlier than blood.
However, that most of the tested tissues became PCR negative could be an indication that the parasite is either suppressed in these tissues, cleared from these tissues or otherwise is leaving these tissues between 2 and 3 weeks post infection.
For antioxidant demonstration in hepatic tissue, clear supernatants were separated and used for analysis: 1.
Following thawing, the sample slides were deparaffinized with Tissue Clear (HistoLab, Göteborg, Sweden).
Paraffin sections (5 μm) were deparaffinized in Tissue Clear (Bayer Diagnostics, Puteaux, France) and rehydrated in a graded series of ethanol.
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