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To avoid degeneration, cancer tissue centres and corresponding cancer-free tissues were snap frozen in liquid nitrogen immediately after excision, handled carefully to avoid contamination with RNase, and stored at −80°C until use.
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Six medial and six lateral healthy menisci (3 paired and 3 unpaired sets of two) were harvested from refrigerated knees of 9 deceased human male donors with an average age of 47 (range, 20 to 61 years), sourced from a tissue banking facility (Comprehensive Tissue Centre, Edmonton, Alberta, Canada).
Scans were shown at standard soft tissue kernel and window (window centre, 20 Hounsfield units (HUs); window width, 360 HU) to avoid pixel averages from surrounding lung parenchyma.
At present, great attention has been placed on the seed and seedlings, based on the Dongmen project results; 3 eucalypt tissue culture centres and 13 centralised nurseries have been established.
Informed consent and ethical approval for the collection of data relating to the Human Liver Cohort was obtained from tissue resource centres at Vanderbilt University, the University of Pittsburgh and Merck Research Laboratories.
Imaging features of perinephric fibrosis exhibit a soft tissue infiltrate centred around the great vessels, with possible extension into the perinephric space and rarely into the retrorenal space and retromesenteric spaces [37].
Young banana (Musa acuminata L. AAA group, cv. Brazilian) seedlings were obtained from the banana tissue culture centre (Danzhou, Institute of Banana and Plantain, Chinese Academy of Tropical Agricultural Sciences).
To capture genetic gains through mass vegetative propagation of superior individuals and families of pure and hybrid breeds, a tissue culture centre was set up in March 1993 at Dongmen.
TF-1 human erythroleukemia cells obtained from American Tissue Culture Centre (LGC Promochem, Middlesex, U.K) were cultured in media as supplier required.
Flow Cytometry and Cell Sorting Shared Resource; Ms. Jennifer Peters of the Cell and Tissue Imaging Centre; Dr. Christopher Calabrese and staff of the Small Animal Imaging facility; and all technicians and staff associated with Department of Surgery, St . JudeChildren's Research Hospital, Memphis, TN 38105, USA.
OSCC cell lines were purchased from American Tissue Collection Centre (ATCC) CRL-16233) and were maintained in Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F12 (DMEM/F12) (Sigma-Aldrich, USA).
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