To avoid degeneration, cancer tissue centres and corresponding cancer-free tissues were snap frozen in liquid nitrogen immediately after excision, handled carefully to avoid contamination with RNase, and stored at −80°C until use.
Scans were shown at standard soft tissue kernel and window (window centre, 20 Hounsfield units (HUs); window width, 360 HU) to avoid pixel averages from surrounding lung parenchyma.
Young banana (Musa acuminata L. AAA group, cv. Brazilian) seedlings were obtained from the banana tissue culture centre (Danzhou, Institute of Banana and Plantain, Chinese Academy of Tropical Agricultural Sciences).
TF-1 human erythroleukemia cells obtained from American Tissue Culture Centre (LGC Promochem, Middlesex, U.K) were cultured in media as supplier required.
Flow Cytometry and Cell Sorting Shared Resource; Ms. Jennifer Peters of the Cell and Tissue Imaging Centre; Dr. Christopher Calabrese and staff of the Small Animal Imaging facility; and all technicians and staff associated with Department of Surgery, St . JudeChildren's Research Hospital, Memphis, TN 38105, USA.
OSCC cell lines were purchased from American Tissue Collection Centre (ATCC) CRL-16233) and were maintained in Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F12 (DMEM/F12) (Sigma-Aldrich, USA).
Imaging features of perinephric fibrosis exhibit a soft tissue infiltrate centred around the great vessels, with possible extension into the perinephric space and rarely into the retrorenal space and retromesenteric spaces [37].
Informed consent and ethical approval for the collection of data relating to the Human Liver Cohort was obtained from tissue resource centres at Vanderbilt University, the University of Pittsburgh and Merck Research Laboratories.
(a) Sagittal, (b) axial and (c) coronal T2-weighted images show hypointense spiculated soft-tissue thickening centred around the right sciatic nerve at the sciatic notch (white arrows).
At MR imaging they usually manifest as hypointense irregular spiculated soft-tissue thickening centred around the sciatic nerve at the sciatic notch on T2-weighted images; small intermingled hyperintense foci, indicating bloody content, may frequently be observed on fat-suppressed T1-weighted images [2] (Fig. 23).
