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Supplementary Figure 4 High-resolution electron microscopy images demonstrate excellent preservation of ultrastructure across the whole imaged tissue block.
After embedding in paraffin wax, serial 4 μm-sections were cut from each tissue block and stained with alcian blue.
In a first step the pia was removed from the tissue block using fine forcipes and the pia-white matter (WM) axis were identified.
The tissue block was also received, and additional sections were cut for CD138 and IgG4 immunostaining.
The median eminence (ME) and a tissue block containing the preoptic area and hypothalamus (POH) were dissected separately.
(Watch a video of a player walking through a tissue block here).
Patients underwent a pretreatment biopsy or consented to make a tissue block available from an earlier biopsy.
Histology was available for six patients, three samples were inadequate for Ki-67 examination and 1 tissue block was unavailable.
The timing of tissue block sectioning followed by their use is paramount in maintaining the quality and reliability of results.
However, to produce a tissue block of 1-mm thickness and measure its viable and necrotic volumes is technically difficult.
Archival tissue block DNA was prepared as described [32].
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