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In addition to using non-AD tissue as a control of specific immunoreactivity with plasma from pABri vaccinated mice, we absorbed the immune plasma with aggregated/oligomeric Aβ1-42 for immunostaining.: In order to prepare aggregated Aβ for absorption, 1 mg of synthetic Aβ 1 42 was dissolve in 200 µL of 1,1,1,3,3,3 hexafluoro-2-propanol (HFP) (Sigma).
A second set of experiments was conducted on an independent set of tissue as a control.
Thus, it was feasible in this study to use fibroadenoma breast tissue as a control when studying calcification in breast tumours [ 20].
Since it was not possible to obtain corresponding normal brain tissue as a control for tumor DNA, we used blood DNA of same patient as normal DNA.
Each carrier had a grid of 4 × 5 holes and, in addition to the breast tumour tissue samples, contained one core of normal breast tissue as a control.
For the tongue wound, again using unwounded matched tissue as a control, the overall number of significant differentially expressed probe sets was 19,817, which is slightly less than the overall number of significantly expressed gene sets for skin.
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We also amplified β-actin from these same tissues as a control (Fig. 1A).
However, the Rosenwald dataset that we analyzed did not include healthy lymphatic tissues as a control.
3. When comparing differentially expressed clones between quartiles 1 and 4 of PKC-b expression, it would be useful to compare both quartiles to healthy lymphatic tissues as a control.
Normal adult testis tissue as a positive control and appropriate negative controls were included for each case.
Immunostaining for the basal cell marker p63 present only in benign tissue served as a control for accurately distinguishing benign and tumor tissue.
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