We applied WGCNA to brain tissue (App-Ps1, aged, Me7, and rTg4510) and pure microglia (aged, App-Ps1 and Ercc1) datasets, to generate two parallel consensus networks.
Among these transcripts, some are known to undergo AS events in hematopoietic tissues, such as EZH2 [4], or in other tissues such APP [15] and TPM1 [16].
Differentially expressed gene lists from brain tissue datasets: App-Ps1 and control frontal cortex, rTg4510 and control brain tissue, aging brain tissue, ME7 inoculation hippocampus with and without LPS and ME7 and mock inoculation on different time points were used as input for userlistenrichment function.
The aim of this study was to investigate the characteristic morphological alterations of lung tissue after experimental App challenge using computed tomography in pigs.
For antigen retrieval of sections double-stained for PrPres plus CD31 or APP, tissue sections were soaked in citrate buffer, pH 6.0, and heated to 120˚C at 20 lb/in for 20 min using a BioCare decloaking chamber.
Probably this latter system provides much more precise information on the lung damage, but removal of lung tissue affected by App-like lesions in cases of pleuritis can be difficult.
Moreover, the lack of observed increases of trisomy 21 in sporadic AD are consistent with prior studies in both peripheral non-brain and intact brain tissues, which reported APP levels approximating 2N (Podlisny et al., 1987; St Getrge-Hyslop et al., 1987; Tanzi et al., 1987; Bertram et al., 2010), and is further consistent with mosaic CNVs observed here.
To analyze the tissue-specificity of transgenic APP in different brain regions, hippocampal (H) as well as cortical (C) proteomes of APP23 mice were investigated.
To determine, if the transcriptional profiles associated with primed microglia are preserved in mouse brain tissue, expression sets of App-Ps1, aged, rTg4510 and ME7 prion infected mice (-/+ LPS) were analyzed.
We observed that despite the high levels of pCREB in whole tissue lysates of MeA in APP mice, the levels of pCREB in nuclear fraction of the tissue was lower in APP mice compared to control mice and xamoterol increased pCREB level in the nuclear fraction of the MeA in APP mice (Fig. 5D).
Many recent studies of the effects of amyloid-β protein (Aβ) on brain tissue from amyloid precursor protein (APP) overexpressing mice have concluded that Aβ oligomers in the extracellular space can profoundly affect synaptic structure and function.
