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The algorithm quantified the alignment of fibers by comparing each angle with every other from an image such that we gave parallel fibers a score of 1 and perpendicular fibers a score of 0. Two perpendicular lines have an alignment of 0; therefore, in a random arrangement, total alignment = 0.5, and in perfectly aligned tissue, alignment = 1.
As meso boundary aspect ratios were increased, meso-scale cues overrode micro-scale cues and controlled tissue alignment, with a characteristic critical width (∼500 μm) similar to boundary dimensions that exist in vivo in highly aligned tissues.
Our present results were consistent with these observations, and support the idea that the frequency of intracellular calcium elevation is increased by the formation of a boundary between two distinct tissues, such as between notochord and somitic mesoderm, which resembles the tissue alignment seen in the dorsal region of the normal embryo.
Alignment statistics for each tissue alignment.
The patients' position was adjusted with an initial automatic bone alignment, followed by a soft tissue alignment using the prostate-rectum interface.
Additionally, we use cases in which alignment quality is significantly better in the original tissue alignment, both in terms of sequence and topology, to quantify an upper bound on the alignment p-values.
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Planning CT and the verification CBCT were registered first with automatic bony registration, followed by a manual registration based on the soft-tissue alignment and prostate position in the CBCT.
We also screened each tissue pair alignment for recombination, which could potentially obscure detection of compartmentalization.
Final checks of soft tissue tension, alignment, patellar tracking, and range of knee movements is made before wound closure.
Apart from being an important requirement for functional skeletal muscle tissue, such alignment may also potentially enhance myotube striation by restricting cell spreading to suppress myoblast proliferation and to promote cell fusion and skeletal muscle differentiation.
Although these measures of pathway activation were expressed in different tissue, substantial alignment between gene expression in blood and brain biopsies has been shown in Parkinson's, Alzheimer's and Huntington's [ 48– 50] supporting the relevance of this profiling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com