Due to tissue aberration and scattering induced wavefront distortion, the axial resolution could be degraded to around 1 µm [ 24].
It is worth noting that many of these methods focus on tailoring the incident laser beams to reduce the scattering effect (including the tissue induced aberration and refractive index mismatch) of the turbid sample on the light waves.
FISH represents the gold standard for gene copy number analysis, because FISH is independent of the purity of cancer tissue and chromosomal aberrations such as polysomy.
The tissue-specific heritable aberrations in histone methylation pattern could be a reason for the propagation of the trans-generational insulin-resistant phenotype in gestational diabetes (Devaskar and Thamotharan 2007).
These findings highlight the importance of assessing hiPSC-derived tissues for genomic aberrations, however it is envisaged that efficient enucleation during terminal differentiation of erythropoiesis will help to negate any safety concerns that may arise from such somatic or acquired mutations.
The apparent absence of significant T2D-related differential DNA methylation in blood raises the possibility that T2D-related epigenetic aberrations are tissue-specific although more tissues will have to be screened to substantiate this.
Thorough characterization and classification of tissue culture induced chromosome aberrations have led to a better understanding of somaclonal variation (Lee and Phillips 1988; Bairu et al. 2011).
