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We describe the construction and operation of an atomic force microscope based on a tuning fork sensor with probe tips obtained from commercially available cantilevers.
Single-hyphal tips obtained from the fungus growing on water agar were cultured on PDA and stored in sterile distilled water in test tubes at room temperature (Boesewinkle 1976; Ko 2003).
Semi-quantitative and quantitative methods were used for the culture of catheter tips obtained from newborns.
Root tips obtained from germinating seeds of the passion fruit accession 'IAPAR-123' were pre-treated in 2 mM 8-hydroxyquinoline for 5 h at 18°C, fixed in ethanol-acetic acid 3 1 (v/v) and stored in fixative (−20°C).
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C18 zip-tip pipet tips were obtained from Millipore to desalt samples for MS analysis.
Net genetic distances based on the proposed GTR + Γ + I [ 41] model of sequence evolution were calculated on the whole analyzed mtDNA fragment, exporting tip-to-tip distances obtained from Maximum-Likelihood phylogeny calculated in TREEFINDER[ 42].
Root tips were obtained from cultivated materials from greenhouse of Academic Sinica.
The automated liquid handler robot (Biomek FX), as well as regular (AP96 P250) and conductive (Span8 pipetteipetipstips, were obtained from Beckman Coulter.
The tip displacement obtained from the finite element analyses agrees with the measured data very well.
The longitudinal strain, ε c, in the internal crack arm behind the crack tip was obtained from Eq. (7).
When compared in terms of the selection parameter σ∗ of the dendrite tip, data obtained from ECHM fitted better to the σ∗ ∝ δ7/4 relationship over a wider range of δ than those obtained from CPFM.
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