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The physiological role of CAT8 may thus be in equilibrating the tip tissue with Gln to supply all cells with assimilated nitrogen for growth.
Following the established methods [32], antler tip tissue for mRNA extraction was rapidly separated into five morphologically defined layers (Fig. 4) under a dissecting microscope, snap frozen in liquid nitrogen and stored at −80°C.
For shoot tip tissue, seeds were surface sterilized, scarified with sandpaper, and germinated on moistened filter paper in Petri dishes in a growth chamber with the following conditions: 16 h high intensity light (21°C), 8 h darkness (21°C).
Fresh stem tip tissue was used for all size estimates reported.
Apparantly, the ASGV titer in the meristem tip tissue decreased along with increasing treatment times at high temperature.
This shows that the changes in mRNA levels in stem base tissue and in shoot tip tissue are different.
Similar(45)
Abdominal tip tissues were removed and the solvent was evaporated under a gentle N2 stream.
The greatest difference in expression pattern was observed between the tip tissues and YL.
In addition, the other target genes showed the opposite expression patterns in base tissue compared with tip tissues (Fig. 8 and Table 3).
The two tip tissues (RT and ST) had similar genome expression patterns, and so did the two internode tissues (RI and SI).
Small RNA extraction was initiated from 200 mg of a mixture of leaves, stem or root tip tissues from 10too 100 seedlings for each time point.
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