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The tip of application pipette was positioned ∼15 µm from the recorded neuron.
In these experiments, the tip of application pipette was positioned within ∼15 µm from the recorded cell soma (white arrows, Figure 1A) and brief (1 2 s) puffs of choline were delivered every 2 3 min. Pressure-applied 0.5 1 mM choline (∼5 psi pressure) elicited either no responses (Figure 1C) or small whole-cell currents (<30 pA in amplitude, Figure 1D).
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We inject 12 20 ml of a 2.5 mmol/l solution of a gadolinium chelate following fluoroscopically guided puncture of the glenohumeral joint via a classic anterior approach and verification of the intraarticular position of the needle tip by application of a small amount of iodinated contrast media.
The method is based on an asymptotic expansion of the stresses in a small region around a notch tip and application of the conventional BE in the remaining region of the structure.
In keeping with their mission the founders produce a weekly podcast "About Admissions" with lots of tips for applications.
This solution was drawn into the tip of the microelectrode by application of suction to the basal end of the pipette.
The company was apparently doing well and the Yap Voicemail app was most likely the tip of the iceberg, an application of more important IP under development since 2006.
With the sharp tip of the smallest tubulator (application rate of 25 t ha 1; depth of 5 cm) the draught forces in the field were from 520 to 1080 N, depending on soil.
After only a few seconds of application, the distal tip of the fiber-optic bundle was placed in direct contact.
For example, a 30-min pulse application of cytochalasin D broadened the tip of growing hairs, but after the application the tip was quickly recovered to the original growth direction [14].
Each success factor is accompanied by tips for deployment and a real-world example of application.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com