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"isoform 1 is not required for initial tip density formation... .......
Isoform 1 is localized in proximity to the stereocilia tip density.
These data are consistent with isoform 2 being necessary to form the tip density complex and drive stereocilia elongation.
Note: the smaller insertional tip density (arrowheads) can be still observed in P8 Myo15 shairh2 hair cells (F, H ). Scale bars are 200 nm.
The tip density was prominent in all rows of Myo15 ΔN/ΔN stereocilia, indicating that isoform 1 was not necessary for formation of this critical structure.
Branching morphogenesis of the ureteric bud was altered, with expanded tips and reduced tip density, probably contributing to the smaller size of the mutant kidney.
Similar(48)
No significant difference in ureteric bud-tip density was seen between Pygo2+/- and Pygo2+/+ embryos (P = 0.33).
The root-tip density, ρε, of laterals of the lower-order, woody root axes was 0.3 1.0 per cm of root length, whereas ρ ε* of the swarming, higher-order root fans was 10 times greater.
The stereocilia of shaker2 mutant mice lack any tip-density while in whirlin-deficient stereocilia tip-density is reduced to a few patches indicating that myosinXVa may form a scaffold, which is necessary for whirlin localization at the tip [2], [27].
We have now additionally examined the tip- density in Myo15 ΔN/ΔN hair cells at P3, and found that the tip-density is also detected at this younger age (see Author response image 1).
Our current manuscript shows that the tip-density is clearly present in both wild-type and isoform 1-null Myo15 ΔN/ΔN hair cells at P7.
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