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The stringent selection works well for genome data and frequently occurring elements, and provides a reliable TinT pattern.
The TinT pattern from the Tasmanian devil is reasonably similar to those previously observed in the opossum and wallaby [ 35].
To derive the most reliable TinT pattern, all available sequences of selected species should be downloaded from genome (ftp://ftp.ncbi.nih.gov/genomes/ or trace databases ftp://ftp.ncbi.nih.gov/pub/TraceDB/).nih.gov/pub/TraceDB/
However, for the human genome, the minimal amount of data that is necessary under stringent conditions to retain a full TinT resolution is about 10% of the genome, for instance about 300,000 traces are sufficient to receive the representative full TinT pattern.
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The TinT patterns of element activities fit well to the sequence-based reconstruction of the evolution of Alu elements (Additional file 3) and to the commonly accepted phylogenetic tree of primates.
Furthermore, the TinT patterns are largely congruent among these four genomes, suggesting activity of most CR1 subfamilies in the common ancestor of crocodilians, while only few subfamilies were active since the divergence of Alligatoridae and Longirostres.
Three implications can be drawn from the TinT patterns of Alu SINEs: (1) several subtypes of Alu elements were active during overlapping periods, (2) a significant change in Alu activity took place after Tarsius separated from a common ancestor with anthropoids, and (3) the TinT activity profiles correlate perfectly with the well known activity patterns of Alu elements [ 14].
The TinT activity pattern is then graphically displayed.
Therefore a direct comparison to our TinT activity pattern of Alu elements is limited.
Thus, an analysis of the patterns of nested CR1 elements (transpositions in transpositions that we call the TinT method, Figure 1) provides a relative timetable of active CR1 elements.
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