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For example in human cell lines, Rivera-Mulia et al.11 report that developmental shifts to later timing precedes transcriptional down-regulation and developmental shifts to earlier timing follows transcriptional up-regulation for the majority of replication timing switching genes, suggesting that replication timing may be both a driver and a passenger of transcription.
Using MATLAB programming, dynamic optimization processes were done to evaluate the option values of investments and the timing switching technologies.
High confidence replication timing switching domains were selected with a q-value cutoff of 0.01, corresponding to an overall FDR of 1%.
The fraction of chromosome domains that displayed EtoL switching in response to BAF250a loss (commonly misregulated in BAF250a-deficient ESCs and OSKM cells), showed a very similar tendency of replication timing switching in Brg1 but not BAF53a mutant ESCs.
Indeed, we identified a set of chromosomal domains that undergo replication timing switching in BAF250a-deficient OSKM cells and found that significant fraction of these switching domains overlap with those identified in BAF250a-deficient ESCs.
In order to determine the significant replication timing switching domains that are independent of changes between replicates, we determined Euclidian distance at 10,974 200-Kb segments between groups (that is, mock versus OHT) and within groups (that is, mock replicate-1 versus mock replicate-2), wasch was used to calculate P values at each 200-Kb genomic segment.
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Individuals responded to a visual cue by depressing a timing switch.
The downregulation of this transcription may trigger EtoL replication timing switches and cause the heterochromatinization of the corresponding domains, thus contributing to loss of pluripotency.
The time required to reach the target position also depends on the timing for switching the traveling direction.
However, achieving precise control using on-chip miniaturized magnetic coils is challenging and requires delicate combination of operating parameters, e.g., magnetizing current and timing of switching, fluid viscosity, droplet size, etc.
Gray traces show timing of switching between the delivery of clean, de-odorized air (low), and odor-containing air (high).
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