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In general, it is difficult to explain the actual discrepancies in the prevalence of Cryptosporidium spp. and G. duodenalis among different studies because the infection rates are related to many factors, including the examination methods, age distributions of the animals, sample sizes, host health status at the time of sampling, the timing of specimen collection, and geo-ecological conditions.
Perhaps the most critical challenges are the need to determine the type and optimal number of samples, and the proper timing of specimen collection.
The estimated mean human incubation period for monkeypox virus infection is 12 days [5] and the optimal timing of specimen collection for determination of IgM levels using current assays is 7 56 days after appearance of a rash [22].
The timing of specimen collection and delivery is variably tracked.
The importance of the timing of specimen collection should be clearly communicated to healthcare providers.
Information about the timing of specimen delivery to laboratories was not readily available.
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Nosocomial acquisition of drug resistant bacteria is also suggested by the temporal association between i) the timing of specimens that cultured ESBL and MRSA organisms and ii) the duration of current and previous hospital admissions.
The number and timing of specimens collected was left to the discretion of the attending physicians.
Because of the differences in the distribution of age and sex among patient groups and the differences in the number and timing of specimens collected for antibody measurement, we used regression-based modeling approach to examine these factors simultaneously and tried to analyze the relationship between their potential interactions and antibody titers (Table 5, Figure 2A).
As is well known and also seen from our data, reproductive hormones fluctuate over a menstrual cycle, and the timing of the specimen collection is likely to be a critical factor in making comparisons among groups or studies.
Date and time of specimen collection.
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