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Another proposed role for a regulated timing of origin activation is to ensure a reproducible replication end time despite the stochasticity discussed above.
Using our rooted opsin phylogeny, reconciled tree analyses (RTA) provide new information on the timing of origin of the various opsin clades (figure 5).
Here we provide a first report the timing of origin and radiation, long distance dispersal events, and rate of diversification of five of the largest and most diverse Macaronesian plant endemic.
The suppression of the shortened S-phase and the advanced Cdc45 binding in the Δrpd3 mutant upon deletion of SET2 likely reflect a concomitant change in the timing of origin firing, but requires absolute confirmation by a more direct experimental approach, such as 2-D gel analysis.
This timing of origin for cave forms is much earlier than previously suggested (~3.1MYa) [ 10].
Several determinants control the timing of origin firing, including the chromatin environment and epigenetic modifications.
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Even if figure 4A does not represent the true history of opsin, the two viable alternative hypotheses (figures 4B and C) do not drastically alter our interpretation of the timing of origins of animal opsin in RTA analysis.
Two hermit crab families were recovered as non-monophyletic assemblages (Diogenidae, Paguridae), which resulted in multiple timing of origins for these families.
To estimate the relative timing of origins, diversification, body form evolution and transition in habitat, Bayesian molecular dating methods were implemented in BEAST v1.5.2 (Bayesian evolutionary analysis by sampling trees) [ 111].
When comparing timings of origin activation, two equal-sized populations were found, corresponding to two bursts of origins firing (Fig. 3a, Additional File 2).
Thus, the debate of the timing of the origin of introns has shifted to an origin before or after the prokaryote-eukaryote split.
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