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Soft tissue swelling impacts on timing of fixation due to fears of infection and wound dehiscence.
Also, the timing of fixation relative to when/where DNA replication is occurring might contribute to a seeming lack of colocalization, although this does not affect colocalization of RPA, PCNA, or DNA polymerase with BrdU foci.
In separate work, we have also implemented a stochastic rather than a deterministic analysis of this model, and find that although some details concerning the timing of fixation change, the general result is not altered.
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The effects of interest were the timing of the fixation point onsets, the presentation times of the sculptures (C & M; or B & U), and the presentation times of the question mark that cued overt responses.
For the correct timing of the fixation, embryos were continuously scored for each cleavage.
The latter observation, however, did not consider the timing of RNA fixation, which in our series strongly influenced JUN and EGR1 expression.
Using the divergence times established by Johnson et al [16] and Koepfli et al [18] with relaxed molecular clock analyses combined with fossil calibration, it was possible to determine approximate timings for fixation of each of the felid UGT1A6 mutations (Fig. 1 with details in Table 1).
The timing and quality of fixation methods, tissue handling and preparation are critical both in trials and in routine clinical use.
In fact, by analyzing the timing of the mutation fixation, we demonstrated that the increase in growth rate of strain F did not correlate with mutation fixation events, and E. coli clones without any identified beneficial mutation grew significantly faster than the parent strain under ethanol stress (Fig. 5).
The timing of the N2 fixation physiology of Trichodesmium is governed by the circadian clock (Chen et al., 1996, 1998; Dong & Golden, 2008).
Plastic surgeons were involved in the early assessment, and the timing of subsequent debridement, definitive fixation and soft tissue procedures were decided jointly.
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