Exact(6)
To improve the expression level and the solubility of mutant in E. coli, the induction conditions like induction timing, induction temperature, IPTG concentrations, induction time were optimized by an orthogonal experiment (L 25)(5)(4)).
In present experiment, four parameters including induction timing, induction temperature, IPTG concentrations and induction time were investigated by an orthogonal test.
The authors observed that in the case of intensive timing induction phase, compared with standard timing induction, the rate of yeast infections can increase 2.5-fold (8.4% vs. 19%; p < 0.01).
(H ) Average number of APs evoked per stimulus before pairing compared to after pairing for all neurons with negative timing induction (n = 17).
(A ) Mean amplitude of the subthreshold response peak in a single recording with a positive timing induction, for all stimulus positions before and after pairing.
(G ) Average number of APs evoked per stimulus before pairing compared to after pairing for all neurons with positive timing induction (n = 15).
Similar(54)
We therefore calculated the time course of changes in the subthreshold response relative to the time of the pairing spike for all neurons (positive and negative timing inductions, n = 32, Figure 5B, grey lines).
(C ) Shifts in the subthreshold stimulus response center after stimulus-AP pairing, for positive timing inductions (green) and negative timing inductions (red).
In most neurons that received positive timing inductions sensory-evoked spiking rates increased after the pairing protocol.
Citing from the text "positive timing inductions increased the peak voltage amplitude by 21.9 ± 9.0%% (n = 15) or 1.3 ± 0.6 m".
(G ) Increase in stimulus-evoked spiking for neurons receiving positive timing inductions, as a function of distance from the paired position.
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