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Biathlon and cross-country skiing, with athletes competing simultaneously and with a variety of timing checkpoints, require the most complex systems.
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We wanted to test whether this advanced replication timing in checkpoint-deficient cells would also apply to the 8-kb THF described above in a plasmid context.
In other organisms Mps1 kinase is multifunctional, with roles in spindle pole duplication, kinetochore biorientation, mitotic timing, spindle checkpoint signaling, and meiosis [ 7, 8].
"It's a struggle every day to keep this factory going because we have to deal with so many expenses and difficulties with Israeli checkpoints, timing and accurate papers for the drivers.
hSPC105 is required for correct mitotic timing and spindle checkpoint activity.
At 200 nM, hesperadin or reversine each mildly but significantly affected the timing of the checkpoint response to high nocodazole.
For simplicity, we assume that the timing requirement of each checkpoint is the same.
We also formulate constraints to represent the timing requirement of a checkpoint.
However, in this paper, we propose an optimization framework to generate fault-tolerant state-based schedules from the given timing requirements of a checkpoint and the messages that are schedulable.
But for somatic cells, with a much longer S phase driven by a replication timing programme and active checkpoint responses to delay entry into S phase, this might not pose a potential threat.
Although the details of this mechanism remain to be clarified, the dissociation rate of the MCC APC/C complex more than the mechanism per se, modulates the balance of inhibition and release and determines the basis for single kinetochore sensitivity and the timing of spindle assembly checkpoint inactivation.
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