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Secondly, the Ir-based emission allows autofluorescence-free, time-resolved imaging and lifetime mapping in cellulo under two-photon excitation, with submicron spatial resolution, on a timescale orders of magnitude longer than fluorescence.
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This chapter surveys recent technological developments that have enabled all-atom MD simulation on timescales orders of magnitude longer than was previously possible, dramatically extending the range of problems to which the biophysicist can apply these methods.
In z-scan measurements with high repetition rate lasers, which rely on sensing changes in spatial mode, it is challenging to separate the desired n2 Kerr lensing from thermal lensing [ 25]; in the XPMSS method, because thermal diffusion timescales are orders of magnitude longer than the < 1 picosecond timescale of the temporal phase modulation, thermal effects are readily separable.
In addition, the amplitude of vibration is also found to be growing on long timescales, that is, on a timescale of order ε−1.
In addition, they must be designed, constructed, and installed on a tight timescale in order to be ready for operation in 2016.
The times required for these processes to occur are calculated and compared with the experimental timescale in order to establish a specimen design criterion for valid SHPB testing.
In this study, finite element modeling predicted that the skin surface should only be heated on the microsecond timescale in order to avoid significant temperature rises in living cells and nerve endings in deeper tissue.
The octupole parameter (epsilon_{mathrm{oct}} = 0.068 >0.01), which indicates that the system is in the eccentric Lidov-Kozai regime with a timescale of order (t_{mathrm{oct}}sim t_{mathrm{Kozai}}/epsilon _{mathrm{oct}} sim) few tens of kyr. Figure 6 Inner eccentricity evolution.
If there were a characteristic recovery timescale of order 62 My, and random events lowering biodiversity, this could easily produce a periodicity such as observed.
These timescales are orders of magnitude longer than conventional FLIM and thus provide high-sensitivity autofluorescence-free imaging.
These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence-free imaging.
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