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Fish caught from the same location in different years were pooled for genetic analysis because the timescale of sampling was short in comparison with the 50-year life span of black rockfish.
This date was consistent with our clade-specific PCR amplification results; however, the timescale of sampling and rapidity of these outbreaks offer only limited variation across the core genome with which to infer evolutionary and epidemiological processes, and thus may impact the robustness of this analysis.
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Rapidly evolving populations such as viruses and bacteria are of particular interest, as such populations may undergo significant demographic variation over timescales comparable with the age of the genealogy of sampled data.
First, our informativeness profiles show partitions of the fish mitochondrial genome to be more informative at recent timescales, and Miya et al. [ 50] limited their taxon sampling to only 30% of the timescale sampled by Miya et al. [ 40].
31 Data that are collected for a research project can be tailored to the specific populations, timescales, sampling frame and outcomes of interest.
This study provides novel insight into the efficiency of common tendinopathy treatments for arresting and reversing degenerative structural changes in the initial stages of disease Limitations: Conclusions regarding the efficiency of these treatments is limited by the short timescale of the follow-up sample collection.
Given the timescale of this study (15 months of sample collection over a total period of two total years), it is possible that we violated the assumption of demographic closure.
However, there is no evidence that subsequent pregnancy modified the effects of near miss with perinatal loss on social support, financial difficulties or K10 score to any large degree (results not shown), although the sample size and timescale of the study did not permit a thorough investigation of this.
The small timescale and sample size of this study - together with the lack of a control group - make it hard to draw firm conclusions.
Transitions from the unfolded to the folded state did not occur on the timescale of these simulations, despite the expected improvement in sampling of REMD over conventional molecular dynamics (MD) simulations.
The example datasets consist of time series from three experiments (called E1, E2 and E3) of varying timescales and sampling regimes under some mock treatment conditions (see Supplementary Fig. S1 for examples).
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