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With residence times of approx. 1.4 s a yield of 78.5% of the target ester molecule was achieved.
Survey XP spectra were acquired in a single sweep with a pass energy of 80 eV, in steps of 0.35 eV and dwell time of 150 ms, giving collection times of approx. 9 min per spectrum.
The handling of the probe is easy, with measurement times of approx. 5 s per region.
MALDI-QqQ-MS/MS does not require liquid chromatographic separation prior to analyses and thus can result in analysis times of approx. 10 s per sample and is therefore very suitable for the analysis of many samples in a short time.
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The authors claim that they achieved a cheap method for producing small quantities of radiochemically pure 68Ga for 2 4 patients in a beam time of approx. 1 h.
The CT scan was followed immediately by a PET scan performed using a standard whole-body acquisition protocol with six or seven bed positions and an acquisition time of approx. 2.5 min per bed position (adjusted to patient size).
At the first 24 h, both cells grown at the same pace with a doubling time of approx. 24 h.
The 8701-BC cells have a doubling time of approx. 29h and have been maintained in culture for more than 100 passages.
It also compared well to the operational half-life time of approx. 4 h reported for TvDAO under surface-aerated conditions [ 24].
The pulse sequence repetition time and the total data acquisition time were determined by respiratory rate (scan time of approx. 6 min at respiratory rate of 30 breathes per minute).
Analysis times for pemetrexed on HPLC- or LC-MS instrumentations are between 7 and 20 min [ 3, 5, 17] per sample measured by HPLC and up to 30 min per sample for LC-MS [ 18] while the MALDI-QqQ-MS/MS assay has an analysis time of approx. 10 s per sample (Fig. 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com