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Uric acid was measured using a timed end point method on a Beckman Coulter Synchron CX9.
The progression of staining in the early time points reflects the relative pattern at the 18 h time end point.
Serum creatinine was measured using the timed end point method (Unicel DxC 800, Beckman and Coulter, Krefeld, Germany).
The outcome measurement of insulin resistance at the final time end point is cross-sectional and reflects risk at age 14 years.
Urine protein concentration was measured by the timed end point method and urine creatinine concentration was measured by the modified Jaffe method [ 31, 32].
We were compelled to use this time end point in consideration of the major heterogeneity in follow-up duration from one centre to another.
Plasma glucose concentrations were determined using the hexokinase timed end point method on the Beckman Coulter Unicel DxC600 Synchron Clinical Analyzer (Beckman Coulter Inc., Fullerton, CA) with SYNCHRON CX Systems GLUCOSE reagent (Cat#442640).
The hexokinase timed end point method was used to determine plasma glucose levels using the Beckman Coulter Unicel DxC600 Synchron Clinical Analyzer (Beckman Coulter, Fullerton, CA) and SYNCHRON CX Systems GLUCOSE reagent (cat. no. 442640).
After the infections with GI.3 and GII.4, serum antibody levels to both VLPs measured at 18 months increased 16 times (end-point titers 51,200 and 102,400, respectively) compared to the pre-infection titers at 12 months (end-point titers 3200 and 6400) (Fig. 1).
Similar calculations were attempted for the subsequent time end points (18 months and 2 years).
Therefore, predefined time end points were used as fixed censored dates for repeated analyses.
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