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The latex gel samples were diluted several times before testing with doubly distilled water.
Isolates were stored at –70°C and were subcultured to trypticase soy agar plates containing 5% defibrinated sheep blood (BD BioSciences, Sparks, MD, USA) >2 times before testing.
In order to confirm that the tumor samples were still viable during the entire experiments, the viability of the tumor explants was determined at different times before testing the antineoplastic effect of CA, UA, and RA.
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From the total 16 specimens in each group, eight were subjected to μSBS testing after 24 h storage in distilled water at 37°C, while the remaining specimens were subjected to thermocycling for 10,000 cycles at 5 and 55°C, with 25 s dwell time before testing.
Reusable aluminum pots and pans were obtained from a supplier of trekking equipment (see Fig. 2) and were washed three times with tap water before testing.
The confining pressure would then be increased by 10 MPa and the sample would be left to "soak" for the appropriate amount of time before testing the permeability using the method described above.
Well, the times before, we were just testing the water.
The children were allowed to practice the actual motor competence tests for three times before being tested.
Each time before testing, the open field, plus maze, and complex maze were rigorously cleaned with soap and water, and investigators always left the room during the test.
A univariate analysis was conducted by using the Fisher exact test to identify factors influencing test sensitivity (i.e., patient country of origin, severity of disease, number of days after illness onset that sample was collected, and sample storage time before testing); p<0.05 was considered significant.
The method was demonstrated one time at each site before testing to ensure that the participants were familiar with the test.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com