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Several factors need to be considered when employing extraction techniques including the solvent types and ratios, extraction temperatures, extraction times, and solid to liquid ratios to ensure a complete extraction of the compounds of interest, while avoiding chemical modification [31, 33 35].
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We also evaluated the influence of increasing hydrolysis times and solids loadings on the minimum protein loading (cellulase and β-glucosidase) required to achieve efficient hydrolysis.
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The pretreatment conditions were optimized using a response surface methodology (RS M on a 23 full central composites design by varying temperature, reaction time and solid loading.
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A Doehlert experimental design was conducted and surface response methodology was used to determine the effect of temperature, contact time and solid liquid ratio on isoflavone extraction from soybean flour or Soybean Protein Isolate in pressurized water system.
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