In the case of de novo protein synthesis, newly synthesized ABAleon2.1 proteins would not affect the ratiometric measurements in the timeframe of the experiments in Figure 3, because the maturation time of mTurquoise is > 1 h (Goedhart et al., 2010).
In the context of symbiotic initiation, during which there is no V. fischeri proliferation within the timeframe of our experiments, such other sources are less likely to be relevant to the induction of early-stage apoptosis.
These results further indicate that downregulation of JH signalling in adult intestinal progenitors does not compromise their viability or function at least within the timeframe of our experiments—and are now shown in Figure 3 figure supplement 1 panels E-F.
Additionally, the reproducibility of LFQ experiments is also largely based on the timeframe of the experiment [ 14]; therefore, in the present study, we standardized the entire pipeline, from sample collection and processing, to instrument setup and calibration.
The AH1 tetramer with the disulfide trap was not used in these experiments because its fluorescence intensity is not reduced during the timeframe of this experiment.
This interpretation of array data relies on the assumption that each hybridization reaction goes to completion within the timeframe of the experiment and that the behavior of all pairs of intended reaction partners in the experiment is somewhat uniform.
In addition, the concentration of ethyl propionate kept increasing over the timeframe of the experiment, but levels of the other 3 ethyl esters decreased during the stationary phase (Figure 4). Figure 2 Time courses of alcohols and acetaldehyde concentrations at each temperature.
This buffer inhibited murine and human Na+/K+-pump activity as determined by intracellular potassium levels and did not affect cell viability in the timeframe of the experiment (Figure 4f and Figure S6).
However, in the presence of Fe3+ with no competing ligands, [Zr(DFO)]+ is stable within the timeframe of the experiment.
Moreover, within the timeframe of this experiment, they are expected to affect plant growth to opposite directions.
