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The method needs no prior, time-consuming alignment of the proteins and therefore an on-the-fly searching of PDB scale crystal structure database for potential bioisosteric replacements is feasible.
In contrast, PAGAN/free and PaPaRa needed 3.3 and 4.7 min for the most time-consuming alignment.
The most popular method for sequence comparison has been to find local alignment pairings using BLAST [ 4] but due to speed limitations, other software has emerged to bypass the time-consuming alignment step by simply counting the number of short sub-sequences shared between a subject and query.
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First, since automated methods, like RNASalsa [ 89], remain largely underappreciated, secondary structure guided alignment still relies on extremely time-consuming manual alignment.
It means that only about 24 (101,315/4254) bound templates are needed to do the time-consuming structural alignment with the query per prediction.
Thus, to improve the efficiency of our algorithm, Homology Indexing is introduced and then the time-consuming structural alignment will be limited only among homologous proteins.
Although, it is more time-consuming, local alignment is more sensitive to finding smaller query sequences in the target sequences.
Alignment-based approaches so far relied on time-consuming manual alignments and discarded up to 50% of the sequenced nucleotides prior to phylogenetic inference.
The newly reported relationships are at least as congruent with the evidence from the fossil record as those inferred from time-consuming manual alignments after manual exclusion of not unambiguously alignable regions.
As seeds increase in length and decrease in redundancy, their probability of seeding time-consuming spurious alignments decreases but so does their probability of not seeding what would be a legitimate alignment.
The disposable integrated microfluidics with self-aligned planar microlenses allows increasing sensitivity of the device and reducing time-consuming optical alignments.
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