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In so doing, Yarger weaves together events and interactions that belie standard linear time sequencing.
I had a hard time sequencing the vinyl, I wanted the tracklisting to draw you in and tell you a story, so when Tony sent me his tracklisting, I was intrigued as it was very different to how I had approached it.
I'll be listening to a bunch of hip hop for a few weeks and just be making beats, then I'll hear a great house record and I'll spend all my time sequencing 4x4 kicks, then I'll hear a really touching indie rock song and it just goes on and on.
At the same time, sequencing bias was analyzed by detecting the random distribution of reads in the assembled Unigenes.
We used a single molecule real time sequencing method (Pacific BioSystems SMRT; PacBio) to analyze the diversity of the SCRaMbLEd library recovered from E. coli.
Morioka, M. S., Kitazume, M., Osaki, K., Wood, J. & Tanaka, Y. Filling in the Gap of Human Chromosome 4: Single Molecule Real Time Sequencing of Macrosatellite Repeats in the Facioscapulohumeral Muscular Dystrophy Locus.
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A library of single molecule real-time sequencing technology, which yielded 67 Mbp of sequence with a read average of 1.2 kb, was obtained.
Real-time sequencing is faster and uses fewer chemicals, making it much cheaper.
Chin, C.-S. et al. Phased diploid genome assembly with single-molecule real-time sequencing.
Flusberg, B. A. et al. Direct detection of DNA methylation during single-molecule, real-time sequencing.
Single-molecule real-time sequencing technologies generating long reads promise to solve such assembly problems.
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