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Scientists have for the first time reconstituted a complex form of RNA virus from only its matching DNA template.
Roughly at the same time reconstituted in vitro experiments showed that the Arp2/3 protein complex initiated similar actin branches after activation [ 386].
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Fractions were dried, washed two times, reconstituted in 50 µl water and stored at -20°C.
Bacteria were centrifuged at 10,000 rpm for 2 minutes, washed with BHI medium three times, reconstituted with BHI medium, and quantified by spectrophotometry.
But fell, and by the time he reconstituted it was too late.
For each aging time course, reconstituted core complexes were incubated at room temperature (∼22 °C) and protected from light for up to 432 h as specified.
The pellet was washed several times and reconstituted with 10 mL of 50 mM Tris buffer (pH 7.0) containing 1 mM phenylmethanesulphonyl sfluoride (PMSF) as a protease inhibitor and 2 mM of DTT as a reducing agent for protecting the thiol group in the enzyme.
Previously, this period was known as a time of "reconstituting the force" -- but that resonates of returning to a prewar status quo.
Further delay is extremely dangerous, giving Baghdad additional time to reconstitute its germ weapons and nerve gas programs, undetected and unmolested, Mr. Holbrooke, working closely with Washington, must now try to reunify the Security Council behind its own Iraq resolutions.
The very act of this production does that; each time, it reconstitutes itself.
This was meant simply to halt the decimation of the Indian population and to give the surviving Indians time to reconstitute themselves.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com