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Similar levels of transcript accumulation were observed in G. intraradices- and mock-inoculated roots at the latest time point here analyzed (96 hours post-inoculation).
To generate the sequence libraries, equal amounts of DNA from bacterial mass isolated from each biopsy were pooled for each time point, here referred to as Antrum T0 and Antrum T1.
In contrast to changes in gene expression pattern in the first days of treatment, gene expression signatures at a single time point, here at baseline, were not reliable in predicting the clinical outcome.
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Since the DEI tool TRNinfer does not accept large datasets (with many time points), here we use smaller datasets than the previous group of experiments with at most 75 time points.
It has to be mentioned that this gene was detected in comparison (a) as well, but exhibited an at least twofold increased transcription level only in the last three time points (here, seven- to tenfold), hence, it is not listed.
In this model the outcome variable measured at the post treatment time points (here 6, 12 and 18 months) is the dependent variable with outcome at baseline (EDE), treatment (MBT or SSCM), time (12 or 18 months), a treatment × time interaction and stratification factors (BMI and service type) as covariates.
However, we cannot exclude the possibility that the expression of Runx1 may end shortly before the last time point tested here, due to the ß-gal protein possibly having a longer half-life than that of Runx1.
Thus at the time point used here, the zygotic mutant likely contains residual Hb protein in the anterior at the time point we use in this study.
At the 24 h time point sampled here, only minor fluctuations were observed, suggesting acute COX-dependent responses had subsided by this time.
Although by gene array we did not identify significant changes in the expression of bradykinin and substance P at the time point tested here, we found changes in substance P receptor and its precursor.
Figure 5 shows that the photopic ERG response in injected eyes was restored to near wild-type levels in this group and persisted for up to 270 days PI (the longest time point examined here).
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