Exact(3)
At the same time, pellets without inoculation were checked in the same conditions.
With time pellets rounded up and formed spherical structures that floated in the culture media.
At the same time, pellets containing 0.25 mg of 17 β-estradiol (21 day-release) were implanted in each animal.
Similar(57)
At the same time, pellet-like morphology could be preserved better.
The cavity included additional measurement devices to correlate the dielectric properties with the main parameters of the expansion process, such as temperature, expansion time, pellet volume and absorbed power.
To analyze the effect of FtsY- or Ffh-depletion on protein expression, depleted and non-depleted cells were harvested at the indicated time, pelleted by low-speed centrifugation and re-suspended in ice-cold 5% sucrose solution in buffer A (50 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.1 mM DTT, and 1 mM Pefabloc) to the same cell density (A600 ∼10 units).
In these experiments different reaction times, pellet sizes, inlet concentrations and residence times were analyzed.
For electron microscopy, the spermatozoa released from epididymides were washed with PBS for three times, pelleted by centrifugation at 1000×g for 20 min, and fixed in 4% paraformaldehyde plus 2.5% glutaraldehyde in 0.1 M cacodylate.
Aliquots were removed at the indicated times, pelleted, and resuspended in 70% ethanol.
Cells were washed three times, pelleted by centrifugation (1,000 × g for 2 minutes at 4°C), and resuspended in staining buffer.
For synchronization of asexual blood stages, parasite cultures with 3 4% ring stages were centrifuged to obtain the pellet, which was resuspended in five times pellet's volume of prewarmed 5% sorbitol (AppliChem) in RPMI medium (Invitrogen) and incubated at room temperature (RT) for 10 min (Lambros and Vanderberg, 1979).
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