More specifically this implies that the window period is the mean observable threshold crossing time, conditional on assay progression (i.e. actually reaching the threshold).
In this study, chemometric analysis was applied for the first time on photodynamic assays to investigate the cytotoxicity of HY in tumor (HEp-2) and non-tumor (Vero and HUVEC) cell lines.
By incorporating N-Ethylmaleimide and Ni2+ as masking agents, Tb3+/GMP-Cu2+ was further exploited as an integrated logic system and a specific time-resolved fluorescent "turn-on" assay for simultaneously sensing His and Cys was designed.
As the Opera takes ∼10 min to load, image, and unload a plate, the impact of different staining times on the assay performance was assessed.
Besides the reduced hands-on time, this assay allows analysis of herpesvirus entry in semi-permissive and non-adherent cells, which were previously non-assessable but play significant roles in herpesvirus pathology.
Microbicidal/static activity of the aqueous and ethyl acetate extract as well as partially purified phytoconstituents (PPPs) was ascertained by viable cell count method, i.e., time kill assay, on the basis of time taken by compounds to induce complete killing of organisms.
The level of TYMS gene expression was determined by a quantitative RT-real time PCR assay on 5 × 10-μm sections of the FFPE biopsies using ATP5E as a housekeeping gene.
To investigate this, we measured cohesin's residence time on chromosomes using an assay based on the 'anchor-away' technique, as previously described (Haruki et al. 2008; Lopez-Serra et al. 2013).
We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine.
This strategy, called qPCR-HRM, is based on a real time PCR assay (gene dosage quantitative analysis) combined with HRM curves (qualitative analysis of punctual mutations) in a single tube [7].
