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To analyze the relationship between storage time of split donor tissue and outcomes after deep anterior lamellar keratoplasty (DALK) and Descemet's membrane endothelial keratoplasty (DMEK).
In this study, we tested whether it is possible to distinguish between N and water status in spring wheat, at the time of split fertilization (plant growth stage – BBCH 32), by means of spectral reflectance data obtained from a sensor with an effective spectral range of 400 950 nm (i.e. without utilizing the distinct short-wave infrared (SWIR) water bands, which requires a more costly sensor).
Since in our study several DCV isolates were separated from each other by more mutations than from the published isolate, the date of divergence of DCV isolates identified in our laboratory may be similar to the time of split from the EB isolate.
The authors cite the time of split between Rickettsiales and Rhizobiales of 1.5 BYA.
Then, M+1 equations were solved using a bisection algorithm to calculate the estimates of the M+1 parameters (M theta values, one per window, plus the time of split between species measured in 2Ne generations).
Our estimates for θA suggest a bottleneck for both populations at the time of split from the common ancestor, and that this was more intense in the case of A. adalberti.
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The last decade, however, has seen a tremendous advance in our ability to estimate these divergence times; that is, the time of splitting of a group from its closest relatives.
The time of splitting of the N and the C clusters was dated at about 6000 to 8400 years ago.
First, we recorded total number of generations elapsed between its ancestral node and time of splitting into the daughter branches (or tip).
For these conversions, we used a time of splitting between gibbon and great apes of approximately 19.5 million years [ 15, 92- 94] and an assumed generation time of 15 years.
The range and distribution of prior for parameters used to describe these scenarios (effective population size, time of splitting or merging events, and admixture rates) are given as additional file (Additional file 3: Table S2).
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