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MCF7S cells were treated with vehicle alone or singly with various concentrations of 4-OHT and ICI at the time of seeding.
That the inferred pro-neuronal and pro-non-neuronal substates can interconvert explains why there are also some mixed colonies even when RA induction is initiated at the time of seeding the NTERA2.Tom cells.
Cells were induced at the time of seeding.
Cells were reverse transfected with 100 nM siRNA (Dharmacon Research , Inc. at time of seeding using Lipofectamine 2000 (Invitrogen).
At the time of seeding the agar was supplemented with Iressa (0.25 to 2 μM) as described earlier.
DMSO control or BI 2536 (10 n M or 25 n M) was added at time of seeding (5,000 cells/well).
Similar(33)
We suggest that this is due to a lack of knowledge of the exact windspeed at the time of seed release and the poor spatial and temporal resolution of the wind data available to us.
a At the time of seed germination, the embryo cells have abundant storage product deposits.
In orchids, the lignified seed coat could protect the minute globular embryo at the time of seed dispersal.
The number of sampled trees per site ranged from 6 to 12 depending on the availability of reproductively mature individuals at the time of seed collection.
Days to heading (DTH) were calculated from the time of seed soaking to when 50% of the plants flowered in the plots.
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