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We suggest that this is due to a lack of knowledge of the exact windspeed at the time of seed release and the poor spatial and temporal resolution of the wind data available to us.
a At the time of seed germination, the embryo cells have abundant storage product deposits.
In orchids, the lignified seed coat could protect the minute globular embryo at the time of seed dispersal.
Days to heading (DTH) were calculated from the time of seed soaking to when 50% of the plants flowered in the plots.
The number of sampled trees per site ranged from 6 to 12 depending on the availability of reproductively mature individuals at the time of seed collection.
The germination time of seed with TiO2 was reduced to 0.89 days; shoot and seedling length was also increased after treatment of wheat seeds with TiO2 nanoparticles at 2- and 10-ppm concentration.
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Water is applied at the time of seeding and then subsequently only every 10-20 dependingnding on the soil conditions.
At the same time of seeding, 15 pmol of each siRNA was complexed with 2 μl of Lipofectamine RNAiMAX reagent (Invitrogen) and transfected to the cells to a final 25 nM concentration, according to the manufacturer's protocol.
As zero-hour time point the single cell suspension at the time of seeding was used.
MCF7S cells were treated with vehicle alone or singly with various concentrations of 4-OHT and ICI at the time of seeding.
The cells were treated either with vehicle alone or antiestrogens at the time of seeding, and incubated for 7 days as constituting a single passage.
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