The mathematically based information (sedimentation time, kinetics of sedimentation, etc). is essential for an exact evaluation of the preparation of the coating fluid.
Thus, the c(s) distributions suggest that Pep2 gradually associates with the tetramer of TreS in a fast exchange on the time scale of sedimentation.
The fact that two peaks are seen is indicative that the exchange between the tetramer and octamer is slow on the time scale of sedimentation (ca. 10 5 s–10 5
The results of the HYDROPRO calculations are summarized in Supporting Information, SI-Table 4. The sedimentation behavior of MnIII2-Y· NrdF (1 11 μM), NrdE (1 30 μM), and NrdE:FeIII2-Y· NrdF (25 nM to 7 μM) were determined and analyzed using the c(s) model in Sedfit and, due to the detection by the c(s) fits of interactions occurring on the time scale of sedimentation, with Sedphat.
Figure 7a shows the time-dependence of sedimentation.
Figure 5 shows the time-dependence of sedimentation for all three types of spheres.
Fig. 7 Resting-time-dependence of sedimentation ratios of silica spheres with various diameters in a thick BR film.
Fig. 9 Cross-linking time-dependence of the storage modulus G′ (solid lines) and the loss modulus G″ (dashed lines) for a thick BR film Fig. 10 Cross-linking time-dependence of a surface free energy and b shear modulus G for a thick BR film Fig. 11 Resting-time-dependence of sedimentation ratios of silica spheres in a crosslinked BR film.
160 μm BR film thickness; 5 μm silica sphere diameter; resting times: a 5 min, b 30 min, c 10 h, and d 792 h Fig. 5 Resting-time-dependence of sedimentation ratios for silica, PS, and PMMA spheres in a thick BR film.
Even more so, at a laser power of 12 mW, corresponding to the default setting in the FDS systems, on the basis of the ratios of the quantum yield and the apparent photon flux in the geometry in the FDS, for EGFP a bleaching rate of ∼0.1%/h would be expected, i.e. a drop in signal of only ∼ −0.6% during the entire time-course of sedimentation.
