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It is certainly a time of sacrifice.
All the suckling mice had clotted milk visible in stomach at time of sacrifice.
At the time of sacrifice, animals were anesthetized with isoflurane, and the ventral incision was reopened.
The cattle were between 1-4 years of age and unbutchered at the time of sacrifice.
At time of sacrifice, necropsies were performed: the spleen, kidney, liver, and thymus were removed, weighed, and sent for histopathology.
Therefore, our time of sacrifice should yield intermediate leptin concentrations.
Blood samples were obtained at time of sacrifice.
At the time of sacrifice, left tibial length was measured with a micrometer.
Animal body weight was monitored daily until the time of sacrifice.
Tumor weights were determined at the time of sacrifice and necropsy.
Mice from each age group were randomized for the day and time of sacrifice.
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