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As acknowledged by Fry (1990) [ 24], to support the existence of an adaptation cost, the performance of reverted lines on the novel host must be assayed at the time of reversion, and this value should then be compared to that obtained after some generations of reversal, under the same environmental conditions.
Under the null hypothesis that reversion lines did not experience any loss of fitness on the novel host after reversion, the slope of the curve linking performance at the time of reversion to performance at the time of the assay, should not significantly differ between control lines and reversion lines.
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We used predefined timepoints in controlled clinical settings that allow for the linking of genotype data with viral fitness at fixed time of the reversion process within the cohort.
All were admitted to the negative-pressure rooms at the time of smear reversion.
The effects of pH value on the pore structure and response (reversion) time of the sensors are also discussed.
Increase of penicillin G (Pen G) concentration from 600 µg (1000 units)/ml to 6000 µg (10,000 units)/ml in LIM did not appear to affect the ability of the bacteria to form L-form colonies, but increased the reversion time of L-form colonies to classical colonies from 2 days to 5 days.
The lack of reversion over-time of pre-existing viral adaptations in subjects without the relevant HLA type suggest that these changes may require compensatory mutations that do not allow simple reversion, therefore allowing the accumulation of mutations and transmission of pre-adapted viruses.
Euglycemic mice at the time of nephrectomy were followed for reversion to hyperglycemia for confirmation of human islet graft function.
Moreover 18 TB patients, 9 of whom were tested 2 months after acid-fast bacilli (ABF) smear reversion (mean time of therapy 71.25 ± 11.25 days) and 9 of whom were cured of TB (within 12 months of completion of therapy) were also included.
In this paper, the effect of annealing temperature and time on the reversion of strain-induced martensite to austenite in the cold worked AISI 304 stainless steel alloy was modeled by means of artificial neural networks (ANNs).
Tissue- and/or time-specific reversion of the mutation attained by breeding gene trap mutants to lines expressing CreER or CreERT2 in a tissue-specific manner would enable dissection of spatiotemporal requirement of the trapped gene.
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