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The implication is that the persistence time of receptor saturation and effect is more sensitive to duration of elution than to eluted amount.
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Each mode has a distinct mechanical stability and energy landscape that is set at the time of receptor-ligand binding, that is once bound, the conformation of the complex does not change.
These chimeric proteins have been used to determine the site and time course of receptor expression and to relate receptor dynamics with therapeutic outcome.
The effect of the AMPAR diffusion coefficient on the time course of receptor incorporation are seen in figure 1c.
Distribution of scaffold molecules within the PSD has little effect on the time course of receptor capture.
When HER2 was transiently engaged with the humanized antibody trastuzumab, the time course of receptor internalization (Supplemental figure S3 in Additional file 3, panels b through f, red) was similar to that observed in cells engaged with the 300G9 mAb with only minor differences.
Change in distribution of scaffold molecules within the PSD has little effect on the time-course of receptor capture, although the annular distribution displayed a slightly slower rate at later times, past the time point of half saturation.
Our results can be used to estimate the residence time of the receptor on the membrane if interactions with any scaffolding molecules are neglected (the latter are mostly concentrated in a local microdomain called the PSD).
In addition, this short activation time of MET receptor was not sufficient to cause either an invasion or chemotaxis of RH30 cells toward a mouse HGF gradient.
This use of GFP permits real-time analysis of receptor dynamics.
In either case, the release location of AMPARs affects the time-course of receptor incorporation.
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