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At the time of insemination the occurrence of ovulation was checked by transvaginal sonography.
A hypothesis to explain this phenomenon may be that the chronology of events leading to ovulation is different for those animals bred nowadays when compared to what was reported previously; this would result in an inappropriate time of insemination.
Mares received 2,500 IU of human chorionic gonadotropin at the time of insemination and were then evaluated daily to determine the time of ovulation and the amount of intrauterine fluid accumulation (0 = none; 4 = large).
Use of double inseminations is mainly caused by problems to find the optimum time of insemination.
The oocyte was then washed and incubated until the time of insemination in Cleavage medium (SAGE) with 10% HSA.
Blood samples were collected from each cow at the time of insemination and at Day 7 after insemination to determine plasma progesterone concentration.
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Two times of insemination (fixed time AI versus AI at observed oestrus) were compared on the fresh and frozen AI treatments.
The objective was to evaluate the effects of timing of insemination and type of semen in cattle subjected to timed artificial insemination (TAI).
Ovarian ultrasonography was performed at the times of GnRH or hCG and of PGF2α administration, at the time of artificial insemination (AI) and seven days after AI, to determine ovulation.
This study was designed to evaluate whether decreasing circulating progesterone (P4) or increasing circulating estradiol-17β (E2) near the time of artificial insemination (AI) in an Ovsynch protocol would increase pregnancies per AI (P/AI) in lactating dairy cows.
Blood samples were collected from heifers of the Angus (B.taurus) and Brahman (B.indicus) breeds at the time of synchronized insemination (day 0) and were considered representative of the non-gravid cow.
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