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The primary parametric expectation that distinguishes marine vicariance (H 1 ) from colonization (H 2 ) is the relatively small effective population size of the colonized population 2) at the putative time of colonization (τ C ). Secondarily, the possibility of gene flow subsequent to the vicariance event τ V further distinguishes H 1 and H 2, although this assumption can potentially be relaxed.
In a prospective study of hospital patients showed that at the time of colonization, IgG levels were higher in asymptomatic C. difficile colonized patients compared to patients who subsequently developed diarrhea [ 18].
Our primary aim was to identify global changes in bacterial protein abundance over time of colonization that may contribute to this increased host cell attachment.
Furthermore, the lxa-associated proteins are the most likely candidates contributing to the increased attachment observed in both sets of isolates over time of colonization.
This suggests that the observed protein adaptations were the result of altered gene expression over time of colonization, rather than due to altered protein processing.
Africa countries emerged at independence with a complex path dependent set of institutions that were probably even worse than those which they had at the time of colonization.
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The transmission model may easily be estimated with likelihood-based approaches from complete data that consist of the times of colonization and decolonization for each child.
This lack of knowledge may stem from the difficulty to analyze field data on colonization, which generally consist of samples irregularly spaced in time, and lack information about the times of colonization and decolonization.
In addition, the temporal time frame of colonization of South America is congruent with formation of the GAARlandia bridge and the emergence of numerous intermediate islands that formed a pathway between the Nearctic and South America.
Lack of a consistent definition of clearance, uncertainty regarding the time of initial colonization, variation in frequency of sampling for persistent colonization, assays employed and variation in duration of follow-up are limitations of the existing published literature.
Lack of a consistent definition of clearance, uncertainty regarding the time of initial colonization, variation in frequency of sampling for persistent colonization, and variation in duration of follow-up and loss to follow-up all impose substantial constraints on our interpretation of the median time to clearance.
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