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We introgressed this mutation into the Columbia background in order to facilitate its genetic analysis with other flowering time mutants that are in the Columbia background and then used this introgressed line (lov1-4) to investigate LOV1 function.
As individuals get fitter over time, mutants are less likely to retain full fitness or increase their fitness, which is visible as the lowering of the peak at 1 and less pronounced right tails of the distribution, for both types of mutations in the later time intervals.
In crosses between p5cs1 p5cs2/P5CS2, used as male, and Arabidopsis flowering time mutants, used as females, aimed to understand the flowering pathway proline interacts with (Mattioli et al., in preparation), the p5cs1 mutant allele was always transmitted to the outcrossed progeny, while the transmission frequency of the p5cs2 mutant allele was exceedingly low (in average 0.8 ± 0.1%).
In crosses between p5cs1 p5cs2/P5CS2 and flowering time mutants the F1 generation was allowed to self-fertilize and the presence of the p5cs2 mutant allele was assessed from the F2 generation, by sulfadiazine selection or by PCR genotyping of the sulfadiazine resistance gene.
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However, most notably, we show here that with time, mutant skeletal muscles develop a wide spectrum of alterations including atrophy, fibrosis, lipid accumulation and a perturbed regeneration.
This pioneering study generated for the first time mutant organisms which were completely depleted for MMP activity and provided deeper understanding of in vivo roles of individual MMP in the fly development.
Furthermore, to monitor the stability of the background interference, one male and one female wild-type control was included each time mutant mice were processed.
Moreover, at that time, mutant mice showed a substantial loss of HCs (66%) at the cochlear base, mainly affecting OHCs (80%), whereas HCs from the cochlear apex were almost unaffected (Fig 3B, bottom left panels).
To confirm the data and rule out any possible interference of the flowering time mutant genotypes in the transmission of the p5cs2 mutation, reciprocal crosses between p5cs1 p5cs2/P5CS2 and wild type were performed.
However, in cenp-metaΔ mutant cells, while the streaming of Mad2 appeared to be normal, Mad2 signal could still be detected on kinetochores of polar chromosomes by the time mutant cells underwent anaphase, and this signal lasted until late anaphase (Fig. 3B,C; supplementary material Movies 5, 6).
Expression levels of AP2s in the various flowering-time mutants.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com