Examinations of DNA sequences served as the equivalent of an old-time microscope, said Curtis Huttenhower of the Harvard School of Public Health, an investigator for the microbiome project.
At 24,500 times microscope magnification the detailed structure of the GFB reveals that a complex glycocalyx overlies the endothelium, with a high contrast layer sitting above a less electron dense layer just above the fenestrated endothelium.
But other than that it hasn't kept up with the times: microscopes don't really look like this anymore.
That means it can cover a slide in a thousandth of the time a microscope would take.Whether the information thus gleaned will be useful remains to be seen.
Although the underground filmmakers Nick Zedd and Michel Auder moved in the same New York circles for decades, they met and conversed for the first time at Microscope, during a packed retrospective of Mr. Zedd's most controversial films in January.
Indeed, the time the microscope hard- and software requires to switch between bleaching and the acquisition of the first post-bleach image is too long to visualize recovery.
Cells were co-incubated in micro-chambers (Lab-Tek Chambers cover-glasses, Nalgene Nunc, Wiesbaden, Germany) and observed under Zeiss LSM-510 Time Lapse microscope at 37°C and 5% CO2.
PC3 ERK5 or PC3 cells were studied using a Nikon (Melville, NY, USA) TE2000 time lapse microscope ( × 20 magnification).
