Sentence examples similar to time lapse migration from inspiring English sources

Time-lapse migration of average ISQ.

Time-lapse migration of ISQ values was compared with IT groups.

The time-lapse migration experiments were performed using cells seeded at 40 50% confluency.

To assess the effect of FLNb knockdown on random cell migration we used time-lapse migration assays to compare wild-type and FLNbKD cells.

Our time-lapse migration assays allowed us to observe that the major defect associated with loss of FLNa and FLNb was failure to initiate migration rather than a change in mean speed of migration or directional persistence.

However, to validate our model predictions more rigorously, we performed new time-lapse migration studies in which we tracked cell migration on soft and stiff ECMs at a much higher temporal resolution, which in turn enabled us to quantify and statistically analyze trailing-edge protrusion-retraction cycles.

The reduction in area of FLNabKD cells cannot be explained by a delay in cell spreading as images from time-lapse migration assays showed that the cells were fully spread 1 hour after plating and did not spread further over an additional 5 hours (Figure 4D).

The migration was determined using time-lapse cell migration assays.

We found that almost total ablation of either FLNa or FLNb does not significantly affect the ability of HT1080 cells to migrate in time-lapse random migration assays but that shRNA-mediated knockdown of both FLNa and FLNb, or ASB2α-mediated targeting of all three FLNs, leads to defects in migration.

Setup time was typically < 5 min, allowing for several time-lapse cell migration experiments per day.

We assessed adhesion with time-lapse microscopy, migration with transwell assays, focal adhesion kinase and paxillin phosphorylation by western blot, and focal matrix degradation by fluorescent gelatin degradation.