Cells were allowed to adhere for 2 hour before 1 mL of culture medium was added to each of the wells, denoting the starting time at which time-lapse confocal microscopy began.
Time-lapse photography is perhaps the best tool we have to bridge the chasm between the time scale at which plants live and our own.
The time point at which each sample was taken was not dictated by the time that had lapsed between each time point (although this was also recorded), but the DOT in the culture (see Table 5).
The resultant time-lapse video, which runs from 11 July 2011 through 8 August 2011, can be seen at http://timemachine.gigapan.org/wiki/Arizona_Grasslands.org/wiki/Arizona_Grasslands
The time lapse was at least a year, depending on the stage in which participants may have dropped out during the trial.
Time-lapse movies were analyzed for cell division events and the time at which each cleavage plane first appeared was recorded.
To determine if Hsp17.6 expression oscillates we examined the time lapse series from Hsp17.6p GFP roots which had been heat shocked for one hour at 37°C.
After 24 hours, all of the images at each representative location were chronologically converted into a time-lapse video from which cell migratory analysis was conducted.
(A ) Time lapse of a dendritic branch from which a protrusion (red) emerges and collapses.
Time lapse of axon outgrowth at an early stage of culture at the ALI.
