Sentence examples for time in medium from inspiring English sources

After a further 18 h, the cells were replenished with antibody, this time in medium lacking FBS, but containing 10 μg/ml BODIPY® LDL.

To test the proposed method comprehensively, the duration of fault disturbance is varied from 60 ms to 300 ms, considering the typical fault clearing time in medium-voltage distribution system [5].

After that compounds were diluted 100 times in medium and added to the cells (40 μL of cell culture plus 40 μL of compound solution, final concentration of DMSO - 0.5%).

Cells were then centrifuged and washed 3 times in medium prior to analysis or sorting.

Prior to exposure, IPEC-1 cells (∼1×106 cells/well) were washed three times in medium without serum and antibiotics.

The wells were washed three times in medium with 15% FCS before medium with 10% human serum and antigens were added.

On day 9 the cells were washed 3 times in medium and resuspended in 1 ml, and rested over night, before proceeding to a IFNγ ELISPOT assay.

After 10 days, cells were washed three times in medium, and expansion of Vγ9/Vδ2 T cells was assessed using FACScan, as described above.

In 12 cases the cytotoxicity of the TIL was compared with that for TIL from the same tumour after the lymphocytes had been washed a further 6 times in Medium 199.

After removing the NZ, the cells were incubated for various times in medium at 37°C, to allow MTs to regrow, as visualised by immunofluorescence microscopy using an anti-acetylated-tubulin antibody.

After 30 min, cells were washed three times in medium and cultured for 48 hours in 200 μl of conditioned medium (medium supplemented with 10% heat-inactivated fetal calf serum (Bodinco, Alkmaar, The Netherlands), 10% culture supernatant of the EL-4 lymphoma (containing murine IL-2) and 1% non-essential amino acids (Invitrogen)).