Sentence examples for time in buffer from inspiring English sources

Previous studies of TIF have relied on excision of tissue biopsies followed by soaking of the extracted tissue over time in buffer.

DNA from the entire bodies was extracted as a pool using Qiagen's DNeasy kit following the protocol for tissue DNA with an extended incubation time in buffer ATL (3 h at 56°C).

For spectral measurements, the obtained solution of PS-PPO-PNIPAM hybrid NS was dissolved 100 times in buffer.

Between incubations, specimens were washed two to four times in buffer (50 mmol/L Tris-HCl, 300 mmol/L NaCl, 0.1% Tween-20, pH 7.6).

The rest of the mitochondrial pellet was washed three times in buffer B and resuspended in 500 µL of buffer C with 0.3 1% Tween 20; this mitochondrial fraction was used for immunoprecipitation (IP).

Samples were then washed three times in buffer containing 0.5 M NaCl and 0.5 M Na2HPO4, pH 8.3 and counted.

Suspension of cells was washed three times in buffer solution, adjusted to the concentration of 10/ml and then stained according to the manufacturer instruction.

The RBCs are incubated at 37°C by 30 min. After incubation, the RBCs are washed three times in buffer A to remove the extracellular dye.

Cells were stained sequentially with primary and secondary antibody, washed three times in buffer between incubations by centrifugation for 3 minutes at 350 × g and 4°C.

After the sections were washed several times in buffer, they were mounted onto slides and coverslips were applied with Fluormount G (Southern Biotechnology Associate, Inc.; Birmingham, AL).

The band containing the proteins was manually excised, washed three times in buffer containing 50%% acetonitrile and dried using 100%% acetonitrile.