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To estimate viral transduction efficiency, an aliquot of transduced cells was taken from those being used for the transplants and was maintained in culture for 1 week to allow time for expression of the GFP (which is not expressed immediately after transduction, making it difficult to estimate the transduction efficiency directly prior to transplantation).
It was a time for expression of total solidarity with the LGBT community and the city of Orlando, as well as yet another moment to ask why military-style assault weapons are so readily available in our country.
Our present study provides evidence for the first time for expression and transport activity of SVCT2 in the blood-brain barrier in vivo.
Cells were harvested at least 48 hours after transfection to ensure adequate time for expression and association of KCNEs with HERG.
To obtain MII eggs expressing exogenous proteins, oocytes were transferred to dbcAMP-free MEMα medium at 5 hours after microinjection and then cultured for another 15 hours so that the total time for expression was identical to that for GV oocytes (20 hours).
Combined bioinformatic and experimental results indicate that a significant portion of these pseudogenes (45%) are transcribed and we provide evidence, for the first time, for expression of alternatively spliced pseudogene RNAs in the human genome.
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The roots were harvested at the appropriate times for expression studies.
Each study set contained seedlings without any treatment to serve as controls, with the roots for each treatment harvested at the appropriate times for expression studies.
This most likely reflects the time required for expression and maturation of the reporter protein.
In a previous transcriptomic study (Schweiger et al. 2013) we used very early and late time points for expression analysis (8, 24, and 72 hpi).
Important criteria determining the suitability of a viral vector for neuronal tracing are the time course for expression, the intensity of expression, and the morphological detail provided by the labeling.
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